Invitrogen™ PAPD5 Polyclonal Antibody

Goat Polyclonal Antibody

Supplier:  Invitrogen™ PA5142750

Catalog No. PIPA5142750

Description

Additional Information: This antibody is expected to recognize both reported isoforms (NP_001035374.2; NP_001035375.2). This antibody is tested in Peptide ELISA: antibody detection limit dilution 1:32,000.

Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation (PubMed:21788334, PubMed:30026317). Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro) (PubMed:21788334, PubMed:21855801). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1 (PubMed:28383716). Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion (PubMed:18172165). Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation (PubMed:25049417). Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation (PubMed:22442037). [UniProt].

Specifications

• Antigen: PAPD5
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PAPD5
• Gene Alias: 5730445M16Rik; 5830428A09; GLD4; Non-canonical poly(A) RNA polymerase PAPD5; PAP associated domain containing 5; PAP-associated domain-containing protein 5; PAPD5; poly(A) RNA polymerase D5, non-canonical; Tent4b; Terminal guanylyltransferase; Terminal nucleotidyltransferase 4B; terminal uridylyltransferase 3; Topoisomerase-related function protein 4-2; TRF4-2; TUT3; TUTase 3
• Host Species: Goat
• Purification Method: Ammonium sulfate precipitation
• Regulatory Status: RUO
• Gene ID (Entrez): 214627, 307745
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: TBS with 0.5% BSA and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q68ED3
• Gene Symbols: PAPD5
• Immunogen: Peptide with sequence C-TDEVATYRDWISKQ (aa 418-431).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse, Rat
• Product Type: Antibody
• Isotype: IgG