MilliporeSigma™ PAR4, Mouse, Unlabeled, Clone: 14H6,

Catalog No.	Quantity	Conjugate
MABS1299MI	100 μL	Unconjugated

Catalog No. MABS1299MI Supplier MilliporeSigma™ Supplier No. MABS1299

Description

Mouse Monoclonal Antibody

Proteinase-activated receptor 4 (UniProt Q96RI0; also known as Coagulation factor II receptor-like 3, PAR-4, Thrombin receptor-like 3) is encoded by the F2RL3 (also known as PAR4) gene (Gene ID 9002) in human. Protease-activated receptors (PARs) constitute a unique family of seven-transmembrane, G-protein-coupled receptors (GPCRs) activated by proteolytic cleavage of their N-terminal propeptide sequence. Once cleaved off, the N-terminal propeptide fragment functions as a ligand and activates the receptor by binding the second extracellular loop. The four PAR family members (PAR-1 to PAR-4) are widely expressed and activated by multiple proteases, and utilize different types of G-proteins (Gi, Gq, and G12/13) for signal transdution depending on the activating protease and cellular context. PAR-4 is expressed on platelets and exhibits a low-affinity for thrombin. However, PAR-4 is able to form hetero-oligomers with both PAR-1 and the ADP receptor P2Y12 to mediate thrombin- and ADP-initiated signaling. PAR-4 cleavage is significantly enhanced through hetero-oligomerization with PAR-1, and PAR-4 interaction with P2Y12 is directly linked to arrestin-2 recruitment and AKT signaling. PAR-4 is a 7-transmembrane (a.a. 83-103, 109-129, 152-172, 192-213, 248-268, 284-304, 320-343) GPCR activated by thrombin cleavage between R47 and G48, having 3 extracellular loops and 3 intracellular loops between the extracellular N-terminal end (a.a. 48-82) the cytoplasmic C-terminal tail (a.a. 344-385).

Specifications

• Antigen: PAR4
• Applications: Immunocytochemistry, Western Blot
• Classification: Monoclonal
• Clone: 14H6
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: F2RL3, PAR4
• Gene Accession No.: Q96RI0
• Host Species: Mouse
• Immunogen: MBP-conjugated recombinant human PAR4 N-terminal fragment including the propeptide sequence.
• Purification Method: Protein G Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_003941
• Test Specificity: Clone 14H6 recognizes an epitope near (C-terminal) to the thrombin-cleavage site. Unlike clone 5F10 (Mfr. No. MABS1298), clone 14H6 does not protect cell surface PAR4 against thrombin cleavage (Mumaw, M.M., et al. (2015). Thromb. Res. 135(6):1165-1171). Clone 14H6 recognizes prepro- and pro-, but not proteolytically activated, human PAR4. A structual change at the cleavage site following thrombin cleavage is believed to prevent the detection of the activated PAR-4 by clone 5F10.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2b κ

For Research Use Only. Not for use in diagnostic procedures.