Invitrogen™ PAX2a Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143393

Catalog No. PIPA5143393

Description

Store as concentrated solution. Centrifuge briefly prior to opening vial. Positive control: 2dpf zebrafish embryos, 36 hpf zebrafish embryos.

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

Specifications

• Antigen: PAX2a
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene Alias: FSGS7; Opdc; optic disc coloboma; paired box 2; paired box gene 2; paired box homeotic gene 2; paired box protein Pax-2; PAPRS; PAX2; Pax-2
• Immunogen: Recombinant protein encompassing a sequence within the center region of zebrafish Pax2a. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (PFA fixed), Western Blot
• Concentration: 1.21 mg/mL
• Formulation: PBS with 20% glycerol and 0.025% Proclin 300; pH 7
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Target Species: Fish, Zebrafish
• Product Type: Antibody
• Isotype: IgG