Invitrogen™ PAX7 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1117

Catalog No. PIPA1117

Description

Western blot analysis of PA1-117 detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma and skeletal muscle satellite cells and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma and skeletal muscle satellite cells. The ~57 kDa band seems to be enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in ths application. Weak unknown bands of lower molecular weight and at ~110-130 kDa are also detected. Specificity of the antibody was also confirmed in Hela cells overexpressing full length PAX7.

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: PAX7
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PAX7
• Gene Alias: FLJ37460; HUP1; paired box 7; paired box gene 7; paired box homeotic gene 7; paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; PAX7; Pax-7; PAX7 transcriptional factor; PAX7A; PAX7B; RGD1564360; RMS2
• Host Species: Rabbit
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 18509, 5081
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 30% glycerol and 0.05% sodium azide
• Gene Accession No.: P23759, P47239
• Gene Symbols: PAX7
• Immunogen: Purified internal fragment of human recombinant PAX7 expressed in E. coli.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov