PAXX, Rabbit anti-Human, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Supplier:  MilliporeSigma ABE179525UG

Catalog No. ABE179525UG

Description

Anti-PAXX, Cat. No. ABE1795, is a highly specific rabbit polyclonal antibody that targets PAXX (C9orf142) and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Protein PAXX (UniProt: 9BUH6; also known as Paralog of XRCC4 and XLF, XRCC4-like small protein) is encoded by the C9orf142 (also known as PAXX, XLS) gene (Gene ID: 286257) in human. PAXX is a newer member of XRCC4 superfamily that predominantly localizes to the nucleus and is shown to accumulate at sites of DNA damage generated by laser microirradiation. It is involved in non-homologous end joining (NHEJ), a major pathway to repair double-strand breaks in DNA and may act as a scaffold required to stabilize the Ku heterodimer, composed of XRCC5/Ku80 and XRCC6/Ku70, at double-strand break sites where it promote the assembly and/or stability of the NHEJ machinery. PAXX residues 1-113 form a head domain, which is structurally related to those of XRCC4, XLF and SAS6. This is followed by a 31-amino-acid -helix, forming a coiled-coil with the other protomer to make a PAXX homodimer. Two isoforms of PAXX have been described that are produced by alternative splicing. (Ref.: Ochi, T., et al. (2015). Science 347(6218); 185-188).

Specifications

• Antigen: PAXX
• Classification: Polyclonal
• Dilution: Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected PAXX in human prostate, human tonsil, and human cervical cancer tissues.
• Gene Alias: Protein PAXX;Paralog of XRCC4 and XLF;XRCC4-like small protein;C9orf142
• Host Species: Rabbit
• Molecular Weight of Antigen: ∽22 kDa observed; 21.64 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
• Quantity: 25 μg
• Research Discipline: Epigenetics & Nuclear Function
• Test Specificity: This rabbit polyclonal antibody detects PAXX protein. It targets an epitope within 16 amino acids from the C-terminus region.
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH7.4 with 0.05% sodium azide.
• Gene Symbols: C9orf142;PAXX;XLS
• Immunogen: KLH-conjugated liner peptide corresponding to 16 amino acids from the C-terminus region of human Protein PAXX.
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Isotype: IgG