PCDH1 (Protocadherin 1) Monoclonal Antibody (CSTEM31), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
14-915-782	Invitrogen™ 14915782	100 μg

Description

This monoclonal antibody CSTEM31 reacts with human Protocadherin-1, also known as PCDH1 and cadherin-like protein 1. Applications Reported: This CSTEM31 antibody has been reported for use in immunocytochemical staining and flow cytometric analysis. (Fluorochrome-conjugated CSTEM31 is recommended for use in flow cytometery.) Applications Tested: This CSTEM31 antibody has been tested by immunocytochemistry on formaldehyde-fixed and permeabilized human iPSCs, and by flow cytometry on human iPSCs. For immunocytochemistry, this can be used at less than or equal to 5 μg/mL. For flow cytometry, this antibody can be used at less than or equal to 0.5 μg/test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Protocadherin 1, also known as PCDH1 and cadherin-like protein 1, is a transmembrane protein involved in cell-cell adhesion. Protocadherin 1 belongs to a group of cadherin-like proteins termed delta1-protocadherins, which consists of protocadherin-1, -7, -9, and -11(X/Y). The delta1-protocadherins have seven extracellular cadherin-repeats, a transmembrane domain, and a cytoplasmic domain, and can be expressed in two isoforms, short or long. Expression of protocadherin 1 is regulated both temporally and spatially, and plays a role both in early development and mature tissues. In mice, it is expressed in a number of tissues and organs, including the brain, spinal cord, peripheral ganglia, inner ear, hair follicles, kidney, reproductive organs, prostate, and the seminal gland. Notably, protocadherin 1 is a susceptibility gene for Bronchial Hyperresponsiveness in humans. Expression of protocadherin 1 has also been identified on human pluripotent stem cells.

Specifications

• Antigen: PCDH1 (Protocadherin 1)
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with proprietary stabilizer and 0.09% sodium azide; pH 7.2
• Gene Alias: 2010005A06Rik; AI585920; cadherin-like 1; cadherin-like protein 1; PC42; PCDH1; PCDH42; protocadherin 1; protocadherin 1 (cadherin-like 1); protocadherin 42; protocadherin-1; Protocadherin-42
• Gene Symbols: PCDH1
• Primary or Secondary: Primary
• Content And Storage: 4°C, do not freeze
• Gene: PCDH1

• Clone: CSTEM31
• Applications: Flow Cytometry, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: Q08174
• Gene ID (Entrez): 5097
• Isotype: IgG2b κ
• Purification Method: Affinity Chromatography
• Product Type: Antibody