Invitrogen™ PCK1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5120718

Catalog No. PIPA5120718

Description

Positive test controls include: HepG2, Mouse liver, Mouse kidney, Rat liver, Rat pancreas, Rat kidney. The target is usually found in the following locations: Cytoplasm. Immunogen sequence: FGKYLAHWLS MAQHPAAKLP KIFHVNWFRK DKEGKFLWPG FGENSRVLEW MFNRIDGKAS TKLTPIGYIP KEDALNLKGL GHINMMELFS ISKEFWEKEV EDIEKYLEDQ VNADLPCEIE REILALKQRI SQM.

Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.

Specifications

• Antigen: PCK1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PCK1
• Gene Alias: AI265463; GTP; HGNC:8724; MGC22652; PCK; Pck1; Pck-1; PE PEPCKC; PEP carboxykinase; PEPCK; PEPCK1; PEPCKC; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; RATPEPCK
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18534, 362282, 5105
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunoprecipitation, Western Blot, Immunocytochemistry
• Concentration: 2.53 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P07379, P35558, Q9Z2V4
• Gene Symbols: PCK1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 490-622 of human PCK1 (NP_002582.3).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG