Invitrogen™ PCK1 Recombinant Rabbit Monoclonal Antibody (JU84-39)

Catalog No.	Quantity
PIMA534631	100 μL

Catalog No. PIMA534631 Supplier Invitrogen™ Supplier No. MA534631

Description

Rabbit Recombinant Monoclonal Antibody

Positive Control: 293T, HepG2, SH-SY5Y, human liver tissue, human kidney tissue.

Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.

Specifications

• Antigen: PCK1
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: JU84-39
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: TBS with 40% glycerol, 0.05% BSA and 0.05% sodium azide; pH 7.4
• Gene: PCK1
• Gene Accession No.: P35558
• Gene Alias: AI265463; GTP; HGNC:8724; MGC22652; PCK; Pck1; Pck-1; PE PEPCKC; PEP carboxykinase; PEPCK; PEPCK1; PEPCKC; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; RATPEPCK
• Gene Symbols: PCK1
• Host Species: Rabbit
• Immunogen: Synthetic peptide within Human PCK1 aa 26-75.
• Purification Method: Protein A
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 5105
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG