PCNA Antibody (PCNA/694), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2447310.02MG

Catalog No. NBP24473100

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

PCNA Monoclonal specifically detects PCNA in Human samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: PCNA
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P12004
• Gene Symbols: PCNA
• Immunogen: Recombinant full length human PCNA protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
• Content And Storage: Store at 4C.
• Isotype: IgG2a κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: PCNA/694
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: cyclin, DNA polymerase delta auxiliary protein, MGC8367, proliferating cell nuclear antigen
• Host Species: Mouse
• Molecular Weight of Antigen: 36 kDa
• Quantity: 0.02 mg
• Research Discipline: Autophagy, Base Excision Repair, Cell Cycle and Replication, Cellular Markers, Core ESC Like Genes, DNA Polymerases, DNA Repair, Stem Cell Markers
• Gene ID (Entrez): 5111
• Target Species: Human
• Form: Purified

For Research Use Only