PD-L1 B7-H1, Armenian Hamster anti-Mouse, Clone: 10B5, Millipore Sigma™

Armenian Hamster Monoclonal Antibody

Supplier:  MilliporeSigma MABC1131100UG

Catalog No. MABC1131100

Description

Anti-PD-L1 B7-H1, clone 10B5, Cat. No. MABC1131, is an Armenian hamster monoclonal antibody that detects Programmed cell death 1 ligand 1 (PD-L1) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Inhibition Function assay.

Programmed cell death 1 ligand 1 (UniProt: Q9EP73; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274) is encoded by the Cd274 (also known as B7h1, Pdcd1l1, Pdcd1lg1, Pdl1) gene (Gene ID: 50533) in murine species. PD-L1 is a single-pass type I membrane protein that is highly expressed in the heart, thymus, skeletal muscle, and lung. Its expression is also reported on activated dendritic cells, B-cells, and macrophages. Numerous tumor cell lines of lymphoid origin also express PD-L1. PD-L1 expression can be up-regulated by interferon-gamma treatment in monocytes, B cells, and macrophages. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-L1 is initially produced with signal peptide (aa. 1-18) sequence, the removal of which yields the mature protein with a large extracellular (aa. 19-239) domain, a transmembrane domain (aa 240-260), and a cytoplasmic domain (aa 261-290).

Specifications

• Antigen: PD-L1 B7-H1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Purified armenian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
• Gene Symbols: Cd274;B7h1;Pdcd1l1;Pdcd1lg1;Pdl1
• Immunogen: Recombinant fragment corresponding to the extracellular domain of Mouse Programmed cell death 1 ligand 1.
• Quantity: 100 μg
• Research Discipline: Apoptosis & Cancer
• Gene ID (Entrez): NP_068693.1
• Target Species: Mouse
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Inhibition Assays
• Clone: 10B5
• Dilution: Inhibits Activity/Function Analysis: A representative lot blocked the activity of PD-L1 B7-H1 and inhibited apoptosis of activated alloreactive 2C T cells. (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Goldberg, M.V., et. al. (2007). Blood. 110(1):186-92; Joshi, T., et. al. (2009). PLoS Pathog. 5(5):e1000431; Liu, X., et. al. (2016). Sci Rep. 6:36722). Flow Cytometry Analysis: A representative lot detected PD-L1 B7-H1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Dong, H., et. al. (2004). Immunity. 20(3):327-36).
• Gene Alias: Programmed cell death 1 ligand 1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274
• Host Species: Armenian Hamster
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This Armenian hamster monoclonal antibody detects Programmed cell death 1 ligand 1 (PD-L1) in murine species.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.