PD-L1, Mouse anti-Human, Clone: 5H1, Millipore Sigma™

Catalog No.	Quantity
MABC1115100	100 μL
MABC111525	25 μL

Catalog No. MABC1115100 Supplier MilliporeSigma Supplier No. MABC1115100UL

Description

Mouse Monoclonal Antibody

Anti-PD-L1, clone 5H1, Cat. No. MABC1115, is a mouse monoclonal antibody that detects PD-L1 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Programmed cell death 1 ligand 1 (UniProt Q9NZQ7; also known as B7-H1, B7 homolog 1, CD274, PD-L1, PDCD1 ligand 1, Programmed death ligand 1) is encoded by the CD274 (also known as B7H1, PDCD1L1, PDCD1LG1, PDL1) gene (Gene ID 29126) in human. PD-L1 is a single-pass type I membrane protein that plays a critical role in induction and maintenance of immune tolerance to self. It is expressed on activated T- and B-cells, dendritic cells, keratinocytes, and monocytes. Higher expression has also been reported in heart, skeletal muscle, placenta, and lung. PD-1 and PD-1 ligands 1 and 2 (PD-L1 and PD-L2) are B7:CD28 family members that regulate T cell activation and peripheral tolerance. When engaged together with the TCR, the interaction of PD-1 with its ligands delivers an inhibitory signal to T cell proliferation and cytokine production. While PD-L1 is broadly expressed in hematopoietic and nonhematopoietic cells, PD-L2 expression is highly restricted to antigen presenting cells (APCs), including dendritic cells (DCs) and macrophages. The PD-1 pathway plays a key role in the progressive loss of effector T cell responses during chronic HIV infection. Under some conditions, blockade of this pathway can restore many T cell functions. PD-L1 is initially produced with signal peptide (aa 1-18) sequence, which is subsequently cleaved off to produce the mature protein with a large extracellular (aa 19-238) region that contains an Ig-like V-type domain (aa 19-127) and an Ig-like C2-type domain (aa 133-225), followed by a transmembrane domain (aa 239-259) and a cytoplasmic tail (aa 260-290).

Specifications

• Antigen: PD-L1
• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: 5H1
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (Paraffin) Analysis: A representative lot detected PD-L1 in Immunohistochemistry applications (Bigelow, E., et. al. (2013). J Vis Exp. 3;(71); Spranger, S., et. al. (2013). Sci Transl Med. 5(200):200ra116; Parra, E.R., et. al. (2018). Appl Immunohistochem Mol Morphol. 26(2):83-93; Andorsky, D.J., et. al. (2011). Clin Cancer Res. 17(13):4232-44; Sunshine, J.C., et. al. (2017). Clin Cancer Res. 23(16):4938-4944).Western Blotting Analysis: A representative lot detected PD-L1 in Western Blotting applications (Parra, E.R., et. al. (2018). Appl Immunohistochem. Mol. Morphol. 26(2):83-93_.
• Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
• Gene Alias: Programmed cell death 1 ligand 1;B7-H1;B7 homolog 1;CD274;PDCD1 ligand 1;Programmed death ligand 1
• Gene Symbols: CD274;B7H1;PDCD1L1;PDCD1LG1;PDL1
• Host Species: Mouse
• Immunogen: Full-length human recombinant PD-L1.
• Purification Method: Protein G purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Apoptosis & Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_054862.1
• Test Specificity: Clone 5H1 detects human Programmed cell death ligand 1 (PD-L1). It targets an epitope with in the extracellular domain.
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG1 κ