PDE3B Monoclonal Antibody (SMCP3B), eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-129-66	Invitrogen™ 14197380	25 μg
50-129-67	Invitrogen™ 14197382	100 μg

Description

Description: Cyclic nucleotide phosphodiesterases (PDE) catalyze the hydrolysis of cAMP and cGMP thereby regulating the effects of these second messengers. There are several families of PDE which share common structural organization. The PDE3 family has two members, 3A and 3B that are products of two separate genes. PDE3B (∽130 kDa) is expressed predominantly in adipose tissue and lymphocytes, while PDE3A (∽120 kDa) is expressed predominantly in platelets and heart tissue. The monoclonal antibody SMCP3B reacts with mouse PDE3B. The SMCP3B antibody was generated against amino acids 643-1100 in the C-terminus and does not cross-react with mouse PDE3A, as determined by western blot analysis of lysates of mouse heart tissue. The SMCP3B antibody does not cross-react with human PDE3B. Applications Reported: This SMCP3B antibody has been reported for use in immunoblotting (WB). Applications Tested: This SMCP3B antibody has been tested by immunoblotting of mouse lymph node cells and mouse heart under reducing conditions. It can be used at 1-5 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

Specifications

• Antigen: PDE3B
• Concentration: 0.5 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Alias: 9830102A01Rik; AI847709; CGIP1; CGI-PDE B; CGIPDE1; cGMP-inhibited 3',5'-cyclic phosphodiesterase B; cGMP-inhibited 3',5'-cyclic phosphodiesterase B; LOW QUALITY PROTEIN: cGMP-inhibited 3',5'-cyclic phosphodiesterase B; cyclic GMP-inhibited phosphodiesterase B; cyclic nucleotide phosphodiesterase; HcGIP1; LOW QUALITY PROTEIN: cGMP-inhibited 3',5'-cyclic phosphodiesterase B; PDE3B; phosphodiesterase 3B; phosphodiesterase 3B cGMP inhibited; phosphodiesterase 3B, cGMP-inhibited
• Gene Symbols: PDE3B
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: PDE3B

• Clone: SMCP3B
• Applications: Western Blot
• Conjugate: Unconjugated
• Host Species: Rat
• Target Species: Mouse
• Gene Accession No.: Q61409
• Gene ID (Entrez): 18576
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.