MilliporeSigma™ PDI, Rabbit, Unlabeled, Polyclonal,

Catalog No.	Quantity	Conjugate
ABS1655	100 μg	Unconjugated

Catalog No. ABS1655 Supplier MilliporeSigma™ Supplier No. ABS1655

Description

Rabbit Polyclonal Antibody

Protein disulfide-isomerase (EC 5.3.4.1; UniProt P07237; also known as Cellular thyroid hormone-binding protein, p55, P4Hbeta, PDI, Prolyl 4-hydroxylase subunit beta) is encoded by the P4HB (also known as CLCRP1, ERBA2L, PDI, PDIA1, PO4DB) gene (Gene ID 5034) in human. Protein disulphide isomerase (PDI), BiP (GRP78 or heat shock 70kDa protein 5), and calreticulin (CRT) are ER lumen folding factors that, together with other PTM enzymes, assist the process of newly synthesized proteins before their release from ER. PDI, BiP, and CRT themselves are subject to posttranslational signal peptide removal after their synthesis, exposing D18, E19, and E18 at the newly formed N-terminus, respectively. The N-terminus D and E residues of the mature proteins are permissive to arginine-tRNA-protein transferase 1/ATE1-catalyzed arginylation, forming arginylated mature proteins (R-DPI, R-BiP, and R-CRT) with altered cellular localization to allow their participation in non-ER functions. Although only the basal levels of R-PDI and R-CRT, but not R-BiP, are generally detectible in non-stimulated cells, upregulated N-terminal arginylation of all three proteins is observed upon cytosolic dsDNA exposure and proteasomal inhibition, indicating a shared role in innate immune responses to invading microbes. In addition, ER stress induction by thapsigargin treatment synergistically boosts proteasomal inhibition-induced upregulation of cellular levels of R-DPI, R-BiP, and R-CRT, suggesting that ER stress accelerates the supply of ER lumenal DPI, BiP, and CRT for N-terminal arginylation.

Specifications

• Antigen: PDI
• Applications: Immunocytochemistry, Western Blot
• Classification: Polyclonal
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rabbit polyclonal antibody in PBS with 0.05% Sodium Azide.
• Gene: P4HB, CLCRP1, ERBA2L, PDI, PDIA1, PO4DB
• Gene Accession No.: P07237
• Host Species: Rabbit
• Immunogen: Linear peptide corresponding to the N-terminal sequence of arginylated mature human PDI.
• Purification Method: Affinity Purified
• Quantity: 100 μg
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_000909
• Test Specificity: This rabbit polyclonal antibody specifically detected the immunogen peptide, but not the control peptide without arginylation at the N-terminal Asp18 (Cha-Molstad, H., et al. (2015). Nat. Cell Biol. 17(7):917-929).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.

For Research Use Only. Not for use in diagnostic procedures.