Invitrogen™ PDIR Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA522384

Catalog No. PIPA522384

Description

Recommended positive controls: A549, HepG2, HCT116. Predicted reactivity: Mouse (90%), Rat (91%), Sheep (91%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

PDIR (protein disulfide isomerase-related protein) is a novel PDI protein that was originally isolated from a human placental cDNA library. It has been suggested that from its nucleotide sequence, PDIR has three CXXC-like motifs (Cys-Ser-Met-Cys, Cys-Gly-His-Cys and Cys-Pro-His-Cys), which are found in the PDI superfamily of proteins. The CXXC motif is responsible for oxidoreductase activity. PDIR is also shown to have a putative endoplasmic reticulum (ER) retention signal 'Lys-Glu-Glu-Leu' at its carboxyl terminus, indicative of ER resident proteins. Northern blot analysis of PDIR indicates that it is preferentially expressed in cells actively secreting proteins. It has also been shown that expression of PDIR is stress-inducible. Data suggest that PDIR, like other PDIs, has oxidoreductase activity of disulfide bonds and facilitates the protein folding process within the lumen of the ER.

Specifications

• Antigen: PDIR
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PDIA5
• Gene Alias: 2700053F16Rik; AU015525; Pdia5; PDIR; protein disulfide isomerase A5; protein disulfide isomerase associated 5; protein disulfide isomerase family A member 5; protein disulfide isomerase family A, member 5; protein disulfide isomerase-associated 5; protein disulfide isomerase-related protein; Protein disulfide-isomerase A5
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10954, 72599
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.52 mg/mL
• Formulation: 0.1M tris glycine with 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q14554, Q921X9
• Gene Symbols: PDIA5
• Immunogen: Recombinant protein encompassing a sequence within the center region of human PDIR. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov