Invitrogen™ PDIR Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5144006

Catalog No. PIPA5144006

Description

Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human HepG2 whole cell, human Caco-2 whole cell, human THP-1 whole cell, human MCF-7 whole cell, human HL-60 whole cell, human A431 whole cell, human T47D whole cell, rat liver tissue, rat lung tissue, rat stomach tissue, rat pancreas tissue, mouse liver tissue, mouse lung tissue, mouse stomach tissue, mouse pancreas tissue. IHC: human breast cancer tissue, human placenta tissue, human gallbladder adenocarcinoma tissue, human rectal cancer tissue, human lung cancer tissue, human ovarian adenocarcinoma tissue, human adrenocortical adenoma tissue. ICC/IF: CACO-2 cell. Flow: U20S cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

PDIR (protein disulfide isomerase-related protein) is a novel PDI protein that was originally isolated from a human placental cDNA library. It has been suggested that from its nucleotide sequence, PDIR has three CXXC-like motifs (Cys-Ser-Met-Cys, Cys-Gly-His-Cys and Cys-Pro-His-Cys), which are found in the PDI superfamily of proteins. The CXXC motif is responsible for oxidoreductase activity. PDIR is also shown to have a putative endoplasmic reticulum (ER) retention signal 'Lys-Glu-Glu-Leu' at its carboxyl terminus, indicative of ER resident proteins. Northern blot analysis of PDIR indicates that it is preferentially expressed in cells actively secreting proteins. It has also been shown that expression of PDIR is stress-inducible. Data suggest that PDIR, like other PDIs, has oxidoreductase activity of disulfide bonds and facilitates the protein folding process within the lumen of the ER.

Specifications

• Antigen: PDIR
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PDIA5
• Gene Alias: 2700053F16Rik; AU015525; Pdia5; PDIR; protein disulfide isomerase A5; protein disulfide isomerase associated 5; protein disulfide isomerase family A member 5; protein disulfide isomerase family A, member 5; protein disulfide isomerase-associated 5; protein disulfide isomerase-related protein; Protein disulfide-isomerase A5
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10954, 360722, 72599
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: Q14554, Q5I0H9, Q921X9
• Gene Symbols: PDIA5
• Immunogen: E.coli-derived human PDIA5 recombinant protein (Position: R46-Y481).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG