Invitrogen™ PDPK1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5144685

Catalog No. FEPA5144685

Description

Immunogen sequence: LDSNSFELDL QFSEDEKRLL LEKQAGGNPW HQFVENNLIL KMGPVDKRKG LFARRRQLLL TEGPHLYYVD PVNKVLKGEI PWSQELRPEA KNFKTFFVHT PNRTYYLMDP SGNAHKWCRK I Antigen sequence identity: Mouse 99%; Rat 99%.

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.

Specifications

• Antigen: PDPK1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Pdpk1
• Gene Alias: 3-phosphoinositide dependent protein kinase 1; 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; mPDK1; OTTHUMP00000174525; OTTHUMP00000205076; PDK1; PDPK1; PDPK2; PDPK2P; Pkb kinase; PkB kinase like gene 1; PkB-like 1; PRO0461; Protein kinase B kinase
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5170
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.05 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: O15530
• Gene Symbols: Pdpk1
• Immunogen: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence. Antigen range: 419-539. Recombinant protein control fragment (Product #RP-109982).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Format: Liquid