MilliporeSigma™ PECAM-1, Rat, Unlabeled, Clone: 390,

Catalog No.	Quantity	Conjugate
CBL1337I	100 μL	Unconjugated

Catalog No. CBL1337I Supplier MilliporeSigma™ Supplier No. CBL1337I

Description

Rat Monoclonal Antibody

Platelet endothelial cell adhesion molecule (UniProt Q08481; also known as CD31, PECAM-1) is encoded by the Pecam1 (also known as Pecam, Pecam-1) gene (Gene ID 18613) in murine species. PECAM-1 (CD31) is a 130-kD cell adhesion molecule that is expressed at the junctions of all continuous endothelia, as well as most bone marrow-derived hematopoietic cells, including platelets, monocytes, granulocytes and some lymphocytes. The human PECAM1 and murine Pecam1 genes both produce multiple spliced isoforms with varying lengths in cytoplasmic domain, with the isoform Long being the major form expressed in human tissues and endothelial cells (ECs), and isoform delta 14-15 being the predominantly expressed murine form. PECAM-1 plays an import role in maintaining vascular integrity and function by mediating ECs interactions with pericytes and smooth muscle cells (SMCs). PECAM-1 is initially produced with a signal peptide (human a.a. 1-27, murine a.a. 1-17) sequence, the removal of which yields the mature protein with a large extracellular region (human a.a. 28-601, mouse a.a. 18-590) composed of six C2-type Ig-like domains, followed by a transmembrane seqment (human a.a. 602-620, mouse a.a. 591-609) and a cytoplasmic tail (human a.a. 621-738, mouse a.a. 610-727) that plays an essential role in PECAM-1-mediated cellular signaling.

Specifications

• Antigen: PECAM-1
• Applications: Immunofluorescence, Immunoprecipitation, Neutralization
• Classification: Monoclonal
• Clone: 390
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rat IgG2a in PBS without azide.
• Gene: Pecam1, Pecam, Pecam-1
• Gene Accession No.: Q08481
• Host Species: Rat
• Immunogen: Mouse leukocyte cell line 32D.
• Purification Method: Protein G Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Cell Structure
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_032842
• Test Specificity: Clone 390 immunoprecipitated the characteristic 120-130 kDa protein from L-cells transfected with muPECAM-1∆12,15 and selectively stained murine vessels in acetone-fixed tissue sections (Baldwin, H.S., et al. (1994). Development. 120(9):2539-2553).
• Target Species: Mouse
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.