Gibco™ PeproGMP™ Human BMP-4 Recombinant Protein, PeproTech®

Catalog No. 01-672-608

Supplier: Gibco™ GMP12005ET50UG

Description

Recombinant Protein

Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-beta superfamily of structurally related signaling proteins. PeproTech's E. coli-derived Human BMP-4 is a fully active homodimeric protein consisting of two 107 amino acid subunits, which correspond to amino acids 303-408 of the full-length BMP-4 precursor, as well as an N-terminal methionine. PeproTech Recombinant Human BMP-4 is a 24.2 kDa protein. PeproGMP BMP-4 can be used with PeproGMP VEGF-165 and PeproGMP SCF in CTS StemScale medium for pluripotent stem cell differentiation, specifically for mesoderm and hematopoetic progenitor cell induction. Reconstitute using 5mM HCl, pH 3.0 to a concentration of 0.1-1.0 mg/ml. Additional product information, specifications, handling and reconstitution information are provided on the Certificate of Analysis. All PeproGMP™ products are manufactured using animal-derived component free materials. Product Description/Intended Use: PeproTech GMP recombinant proteins are manufactured for use as ancillary materials by applying applicable principles of GMP and quality control requirements from USP (United States Pharmacopeia) Chapter <1043> Ancillary Materials for Cell, Gene, and Tissue-Engineered Products.

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. This particular family member plays an important role in the onset of endochondral bone formation in humans, and a reduction in expression has been associated with a variety of bone diseases, including the heritable disorder Fibrodysplasia Ossificans Progressiva. Alternative splicing in the 5' untranslated region of this gene has been described and three variants are described, all encoding an identical protein.

Specifications

• Accession Number: P12644
• For Use With (Application): Control, Western Blot Control, Bioactivity
• Format: Lyophilized
• Formulation: Protein with no preservative
• Gene ID (Entrez): 652
• Molecular Weight (g/mol): 24.2 kDa
• Name: PeproGMP™ Human BMP-4
• Purification Method: Purified
• Quantity: 50 μg
• Storage Requirements: -20°C or -80°C if preferred
• Regulatory Status: GMP
• Endotoxin Concentration: ≤0.1 EU/μg
• Gene Alias: BMP; BMP2B; BMP-2B; Bmp2b1; Bmp2b-1; Bmp4; Bmp-4; BOMPR4A; Bone morphogenetic protein; Bone morphogenetic protein 2B; bone morphogenetic protein 4; bone morphogenetic protein 4 preproprotein; DVR4; Dvr-4; MCOPS6; OFC11; ZYME
• Common Name: BMP4
• Gene Symbol: BMP4
• Biological Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells.
• Product Type: Protein
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Sequence: MKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
• Content And Storage: -20°Cor to +80°C if preferred
• Activity: Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells.
• Expression System: E. coli
• Form: Lyophilized
• Purity or Quality Grade: ≥ 98% by SDS-PAGE gel and HPLC analyses.