Invitrogen™ Pepsinogen I Monoclonal Antibody (8003 (99/12))

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA183390

Catalog No. MA183390

Description

MA1-83390 has an affinity of 4 x 1010 l/mol human Pepsinogen I. Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. Mouse anti Human pepsinogen 1 antibody, clone 8003 recognizes human Pepsinogen I, a zymogen or proenzyme secreted by chief cells in the stomach.

PGA5: Pepsinogen 5, group I (pepsinogen A). Pepsinogens are the inactive precursors of pepsin, the major acid protease found in the stomach. Pepsin is one of the main proteolytic enzymes secreted by the gastric mucosa. Pepsin consists of a single polypeptide chain and arises from its precursor, pepsinogen, by removal of a 41 amino acid segment from the N-terminus. Pepsinogen is synthesized in the stomach lining, and hydrochloric acid, also produced by the gastric mucosa, is necessary to convert the inactive enzyme and to maintain the optimum acidity (pH 1-3) for pepsin function. Pepsin is particularly effective in cleaving peptide bonds involving aromatic amino acids. Pepsin shows extremely broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. PGA5 is a member of the subfamily A1 within the pepsin family and is the predominant endopeptidase in the gastric juice of vertebrates. PGA5 is inhibited by ovUS-1, a uterine serpin.

Specifications

• Antigen: Pepsinogen I
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with <0.1% sodium azide
• Gene Accession No.: P0DJD9
• Gene Symbols: Pga5
• Immunogen: Purified human pepsinogen 1.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

• Applications: ELISA, Immunohistochemistry (Paraffin), Radioimmune Assays (RIA)
• Clone: 8003 (99/12)
• Conjugate: Unconjugated
• Gene: Pga5
• Gene Alias: 1110035E17Rik; Pepf; pepsin A; pepsin A-3; Pepsin A-5; Pepsin F; pepsinogen 3, group I (pepsinogen A); pepsinogen 5, group I; pepsinogen 5, group I (pepsinogen A); pepsinogen A3; pepsinogen A5; pepsinogen F; pepsinogen F protein; pepsinogen-3; Pepsinogen-5; Pg5; PGA3; PGA5
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 5222
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid