Perforin Antibody (PRF1/2468) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP275737

Catalog No. NBP275737

Description

Perforin Monoclonal specifically detects Perforin in Human samples. It is validated for ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array.

Specifications

• Antigen: Perforin
• Classification: Monoclonal
• Concentration: 0.2 mg/ml
• Dilution: Immunohistochemistry-Paraffin 1-2 ug/ml
• Gene Accession No.: P14222
• Gene Symbols: PRF1
• Immunogen: Recombinant human Perforin protein fragment (around aa 413-552) (exact sequence is proprietary) (Uniprot: P14222)
• Purification Method: Protein A or G purified
• Research Discipline: Adaptive Immunity, Apoptosis, Immunology, Innate Immunity
• Gene ID (Entrez): 5551.0
• Target Species: Human
• Form: Purified

• Applications: Immunohistochemistry (Paraffin)
• Clone: PRF1/2468
• Conjugate: Unconjugated
• Formulation: 10 mM PBS with No Preservative
• Gene Alias: Cytolysin, FLH2, HPLH2, HPLH2lymphocyte pore forming protein, Lymphocyte pore-forming protein, MGC65093, P1PFN1, perforin 1 (pore forming protein), perforin-1, PFPcytolysin
• Host Species: Mouse
• Molecular Weight of Antigen: 75 kDa
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.
• Content And Storage: Store at 4°C.
• Isotype: IgG2b κ