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Invitrogen™ Periostin (Postn) Mouse ELISA Kit

Sandwich ELISA Kit

Manufacturer:  Invitrogen™ EMPOSTN

Encompass

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Catalog No. EMPOSTN


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Description

Description

The Mouse Periostin (Postn) ELISA quantitates Ms Periostin in mouse serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Ms Periostin. Principle of the method The Mouse Periostin solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Periostin (PN), also designated osteoblast-specific factor 2 (OSF-2), is a disulfide linked protein originally isolated as a osteoblast-specific factor. Periostin is a secreted protein that binds heparin and functions as a ligand for alpha(V)beta(3) and alpha(V)beta(5) integrins. In preosteoblasts, Periostin acts as a cell adhesion molecule and plays a role in osteoblast recruitment, spreading and attachment. Periostin is mainly detected in lower gastrointestinal tract, aorta, stomach, placenta, uterus and breast tissues but is up-regulated in epithelial ovarian tumors and overexpressed in breast cancer. Expression of Periostin is increased by bone morphogenetic protein (BMP2) and transforming growth factor β 1(TGF β 1). Periostin contains a typical signal sequence, followed by a cysteine-rich domain, a fourfold repeated domain, which shows homology with the insect protein fasciclin, and a C-terminal domain.
Specifications

Specifications

ELISA Kit
This ELISA kit shows no cross-reactivity with the following cytokines tested: Axl, BLC, CD27 Ligand, CD30, CD30 Ligand, CD40, CXCL16, EGF, E-selectin, Fractalkine, GITR, HGF, ICAM-1, IFN-gamma, IGF-1, IGFBP-2, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 ra, IL-1 beta, IL-2, IL-2 R alpha, IL-3, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12 p70, IL-17E, IL-17F, IL-20, IL-23, IL-28, I-TAC, MCP-1, MCP-5, M-CSF, MDC, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 alpha, Osteopontin, Osteoprotegerin, Prolactin, Pro-MMP-9, P-selectin, Resistin, SCF, SDF-1 alpha, TPO, VCAM-1.
Biotin
Other Proteins
Q62009
0.05ng/mL to 16ng/mL
ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Anti-mouse Periostin precoated 96-well strip plate, lyophilized recombinant mouse periostin standard, 20X wash buffer, biotinylated antibody reagent, streptavidin-HRP reagent, TMB substrate, stop solution, contains 0.2M sulfuric acid, assay diluent (5X co
Plasma, 2 μL; Serum, 2 μL; Supernatant, 100 μL
1 hr. 20 min.
Mouse
Mouse
A630052E07Rik, AI747096, OSF-2, Osf2, PLF, PN, peri
<10%
Mouse
0.05 ng/mL
Colorimetric
Colorimetric Microplate Reader
96 Assays
Solid-phase sandwich ELISA
HRP
0.66-16 ng/mL
Periostin (Postn) ELISA Kit, 96 Assays, 1 Kit, Mouse Species, HRP Conjugate, Colorimetric Detection Method, Enzyme-Linked Immunosorbent Assay Designed for the Quantitation and Detection of Periostin, Osteoblast Specific Factor (Postn) in Samples Using a M
50706
RUO
2°C to 8°C
4 hr. 45 min.
Periostin, osteoblast specific factor (Postn)
Development
<12%
96 Tests
Plasma, Serum, Supernatant
A630052E07Rik,AI747096,OSF-2,Osf2,PLF,PN
SDS
Documents
Product Certifications
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