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PeproTech PF4 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No. 500P05BT50
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500 μg
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Catalog No. Quantity
500P05BT50 50 μg
500P05BT25 25 μg
50-271-8720 500 μg
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Catalog No. 500P05BT50 Supplier PeproTech Supplier No. 500P05BT50UG
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Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human PF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PF-4 (500-P05) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PF-4. Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P05BT-1MG will be provided as 2 x 500 μg

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).
TRUSTED_SUSTAINABILITY

Specifications

Antigen PF4
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Biotin
Formulation PBS with no preservative
Gene Pf4
Gene Accession No. P02776
Gene Alias chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; Endothelial cell growth inhibitor; Iroplact; oncostatin-A; PF4; PF-4; Pf4a; Platelet factor 4; Platelet factor 4, short form; RATPF4A; Scyb4
Gene Symbols Pf4
Host Species Rabbit
Immunogen E.coli-derived, 7.8 kDa Recombinant Human PF-4 (CXCL4).
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 5196
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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