Invitrogen™ PF4 Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P0550UG

Catalog No. 500P0550UG

Description

AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography employing an immobilized Human PF-4 matrix. Sandwich ELISA: To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human PF-4 (CXCL4). Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990).

Specifications

• Antigen: PF4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Pf4
• Gene Alias: chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; Endothelial cell growth inhibitor; Iroplact; oncostatin-A; PF4; PF-4; Pf4a; Platelet factor 4; Platelet factor 4, short form; RATPF4A; Scyb4
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5196
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P02776
• Gene Symbols: Pf4
• Immunogen: E.coli-derived Recombinant Human PF-4 (CXCL4).
• Quantity: 50 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody