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PeproTech PF4 Polyclonal Antibody, PeproTech®, Invitrogen™
Description
AA Sequence of recombinant protein: EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PF-4 (CXCL4). Anti-Human PF-4 (CXCL4)-specific antibody was purified by affinity chromatography employing an immobilized Human PF-4 matrix. Sandwich ELISA: To detect Human PF-4 (CXCL4) by sandwich ELISA (using 100 μL/well) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human PF-4 (CXCL4) (500-P05BT) as a detection antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human PF-4 (CXCL4). Western Blot: To detect Human PF-4 (CXCL4) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PF-4 (CXCL4) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Specifications
Specifications
| Antigen | PF4 |
| Applications | ELISA, Western Blot |
| Classification | Polyclonal |
| Concentration | 0.1-1.0 mg/mL |
| Conjugate | Unconjugated |
| Formulation | PBS with no preservative |
| Gene | Pf4 |
| Gene Accession No. | P02776 |
| Gene Alias | chemokine (C-X-C motif) ligand 4; C-X-C motif chemokine 4; Cxcl4; Endothelial cell growth inhibitor; Iroplact; oncostatin-A; PF4; PF-4; Pf4a; Platelet factor 4; Platelet factor 4, short form; RATPF4A; Scyb4 |
| Gene Symbols | Pf4 |
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