Invitrogen™ PFKL Polyclonal Antibody

Catalog No.	Quantity
PA551035	100 μL

Catalog No. PA551035 Supplier Invitrogen™ Supplier No. PA551035

Description

Rabbit Polyclonal Antibody

PFKL Polyclonal Antibody detects endogenous levels of PFKL protein.

Phosphofructokinase (PFK), a major regulatory enzyme in all cells of the body, catalyzes the metabolism of sugar, and thereby is pivotal in the production of energy to maintain normal cell function. In human there are three structural loci controlling PFK: M (muscle), L (liver), and P (platelet) type subunits, which are variably expressed in different tissues; human diploid fibroblasts and leukocytes express all three genes. PFK, a tetramer formed by the random association of the products of two separate gene loci to form the five possible tetramers. PFKs of muscle and liver are homotetramers of the M and L subunits, respectively. Red cells have all five isozymes: M4, M3L, M2L2, ML3, and L4. PFK is an allosteric enzyme activated by ADP, AMP, or fructose bisphosphate and inhibited by ATP or citrate. PFK catalyzes the key controlling step of glycolytic pathway. PFK deficiency can present as mild to life-threatening episodic illness. A hallmark sign of this disease is intermittent dark urine, with the color of the urine ranging from orange to dark coffee-brown, which commonly develops following strenuous exercise. The mean red cell PFK is elevated in persons with Down syndrome.

Specifications

• Antigen: PFKL
• Applications: Immunohistochemistry, Western Blot, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.5% BSA, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene: PFKL
• Gene Accession No.: P12382, P17858, P30835
• Gene Alias: 6-phosphofructokinase type B; 6-phosphofructokinase, liver type; AA407869; ATP-dependent 6-phosphofructokinase, liver type; ATP-dependent 6-phosphofructokinase, liver type {ECO:0000255; ATP-PFK; ATP-PFK {ECO:0000255; EC 2.7.1.11; HAMAP-Rule:MF_03184}; K6PL; liver-type 1-phosphofructokinase; Pfkb; PFK-B; Pfkl; Pfk-l; Phosphofructo-1-kinase isozyme B; phosphofructokinase 1; phosphofructokinase, liver; phosphofructokinase, liver type; phosphofructokinase, liver, B-type; phosphohexokinase; phosphohexokinase {ECO:0000255
• Gene Symbols: PFKL
• Host Species: Rabbit
• Immunogen: Synthesized peptide derived from the C-terminal region of human PFK-B.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 18641, 25741, 5211
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG