PHD2 Rabbit anti-Human, Polyclonal, Bethyl Laboratories

Rabbit Polyclonal Antibody

Supplier:  Bethyl Laboratories, Inc A300322AT

Catalog No. 50-155-7379

Description

The recommended shelf life for this product is 1 year from date of receipt.

HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen. In the presence of oxygen, HPHs convert specific prolyl residues in HIF-alpha to hydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at the cellular level, cause the HIF conversion to be reduced so that HIF is stable and there is increased angiogenesis. HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIF alpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex.

Specifications

• Antigen: PHD2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: EGLN1
• Gene Alias: C1ORF12, Chromosome 1 Open Reading Frame 12, EGL 9 homolog of C. elegans 1, EGLN 1 EGLN1, HIF Prolyl Hydroxylase 2, HIF-P4H 2, HIFPH2, P4H2, Prolyl Hy
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54583
• Content And Storage: 4° C
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/ml
• Formulation: phosphate, tris citrate with 0.09% sodium azide; pH 7-8
• Gene Accession No.: Q9GZT9
• Gene Symbols: EGLN1
• Immunogen: Between 1 and 50
• Quantity: 10 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG