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Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0, for molecular, Thermo Scientific™

Phenol-Chloroform-Isoamyl alcohol mixture in the ratio of 25:24:1 (v/v/v), CAS # 136112-00-0, 67-66-3, 123-51-3, is a solution used in the purification of nucleic acids.

Specifications

Chemical Name or Material Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0
Name Note DNase, RNase and Protease Free
CAS 136112-00-0, 67-66-3, 123-51-3, 136112-00-0
Molecular Formula C6H6O
MDL Number MFCD00133763
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Products 3
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Catalog Number Mfr. No. Quantity Packaging Price Quantity & Availability  
AC327110025
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Thermo Scientific Chemicals
327110025
2.5L Glass bottle
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AC327115000
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Thermo Scientific Chemicals
327115000
500 mL Glass bottle
Each for $331.12
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AC327111000
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Thermo Scientific Chemicals
327111000
100mL Glass bottle
Each for $98.62
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Description

Description

This Thermo Scientific brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

General Description

  • This solution contains a mixture of pure chloroform, isoamyl alcohol and phenol saturated with Tris-EDTA, with a stabilized pH of 8.0

Applications

  • Phenol-Chloroform-Isoamyl alcohol mixture solution (25:24:1) is used in the extraction of nucleic acids, DNA, and RNA
  • When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.
  • It is also used to generate DNA minisatellite fingerprints
Specifications

Specifications

Phenol/Chloroform/Isoamyl alcohol (25:24:1), stabilized, saturated with 100 mM Tris-EDTA to pH 8.0
136112-00-0, 67-66-3, 123-51-3, 136112-00-0
MFCD00133763
1.2800g/mL
none detected
Solution
1ppm max.
Glass bottle
1.28
Yellow to Orange
DNase, RNase and Protease Free
C6H6O
2927
14, 7241
94.11
Molecular Biology
1ppm max.
0.08 to 0.12% hydroxyquinoline
Stabilized
7.8 to 8.2 (25°C)
Safety and Handling

Safety and Handling

Suspected of causing genetic defects, Suspected of damaging the unborn child, Causes damage to organs through prolonged or repeated exposure, Causes severe skin burns and eye damage, May cause drowsiness or dizziness, Suspected of causing cancer, Repeated exposure may cause skin dryness or cracking, Toxic if swallowed, in contact with skin or if inhaled

Germ cell mutagenicity (category 2), Reproductive toxicity (category 2), Specific target organ toxicity after repeated exposure (category 1), Skin corrosion/irritation (category 1), Specific target organ toxicity after single exposure (category 3), Carcinogenicity (category 2), Acute toxicity (category 3)

SDS
Product Certifications

RUO – Research Use Only

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