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Thermo Scientific™ Phire Tissue Direct PCR Master Mix, 1,250 Reactions PROMO
Developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
Supplier: Thermo Scientific™ F170L
Description
Features
- Direct PCR reduces time-consuming and expensive DNA purification steps
- Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
- Specially engineered Phire DNA polymerase for extremely short PCR protocol times
- Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel. Suitable for 1250 reactions.
Applications
- Genotyping
- Transgene detection
- Knockout analysis
- Sequencing
Notes
- The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.
Specifications
2X | |
Medium | |
Phire Hot Start II DNA Polymerase | |
Yes | |
1250 Reactions | |
Genotyping, Hot-start PCR | |
1250 Reactions | |
SuperMix or Master Mix | |
DNA |
2X Phire Tissue Direct PCR Master Mix, 10 x 1.25 mL. Dilution Buffer, 2 x 5 mL. DNARelease Additive, 1.3 mL, 25 μM, Control Primer Mix, 40 μL, Nuclease-free Water, 10 x 1.25 mL, 50 μg O'GeneRuler Express DNA Ladder. Store at -20°C. | |
Direct PCR | |
Fast | |
Direct PCR Master Mix | |
Wet or Dry Ice | |
Built-In Hot Start | |
Blunt | |
Tissue |
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