Thermo Scientific™ Phire Tissue Direct PCR Master Mix, 1,250 Reactions PROMO

Developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Supplier:  Thermo Scientific™ F170L

Catalog No. F170LW25

Description

Features

• Direct PCR reduces time-consuming and expensive DNA purification steps
• Available Dilution & Storage protocol allows multiple PCR reactions from a single sample
• Specially engineered Phire DNA polymerase for extremely short PCR protocol times
• Master mix format with premixed gel loading dye minimizes cross-contamination, reduces sample handling, and allows direct loading on gel. Suitable for 1250 reactions.

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Notes

• The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

Specifications

• Concentration: 2X
• GC-Rich PCR Performance: Medium
• Polymerase: Phire Hot Start II DNA Polymerase
• Includes Label or Dye: Yes
• Quantity: 1250 Reactions
• For Use With (Application): Genotyping, Hot-start PCR
• No. of Reactions: 1250 Reactions
• Reaction Format: SuperMix or Master Mix
• Starting Material: DNA

• Content And Storage: 2X Phire Tissue Direct PCR Master Mix, 10 x 1.25 mL. Dilution Buffer, 2 x 5 mL. DNARelease Additive, 1.3 mL, 25 μM, Control Primer Mix, 40 μL, Nuclease-free Water, 10 x 1.25 mL, 50 μg O'GeneRuler Express DNA Ladder. Store at -20°C.
• PCR Method: Direct PCR
• Reaction Speed: Fast
• Product Type: Direct PCR Master Mix
• Shipping Condition: Wet or Dry Ice
• Hot Start: Built-In Hot Start
• Overhang: Blunt
• Sample Type: Tissue