Invitrogen™ Phospho-A-Raf (Ser299) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5105865

Catalog No. PIPA5105865

Description

Antibody detects endogenous levels of A-RAF only when phosphorylated at Ser299.

Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.

Specifications

• Antigen: Phospho-A-Raf (Ser299)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARAF
• Gene Alias: 1200013E08Rik; Araf; A-RAF; A-Raf proto-oncogene serine/threonine-protein kinase; A-Raf proto-oncogene, serine/threonine kinase; Araf1; A-raf-1; AW495444; Oncogene ARAF1; PKS; PKS2; Proto-oncogene A-Raf; proto-oncogene A-Raf-1; Proto-oncogene Pks; raf a; Raf related protein; RAFA1; raf-related oncogene; Ras-binding protein; Ras-binding protein DA-Raf; RP1-230G1.1; serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein; v-raf oncogene homolog 1 (murine sarcoma 3611 virus)
• Host Species: Rabbit
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11836, 369, 64363
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P04627, P10398, P14056
• Gene Symbols: ARAF
• Immunogen: A synthesized peptide derived from human ARAF(Accession P10398), corresponding to amino acid residues around phosphorylated Ser299.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG