Invitrogen™ Phospho-AANAT (Thr29) Polyclonal Antibody

Catalog No.	Quantity
PA1761	50 μg

Catalog No. PA1761 Supplier Invitrogen™ Supplier No. PA1761

Description

Rabbit Polyclonal Antibody

PA1-761 detects phospho serotonin N-acetyltransferase (AA-NAT) from rat tissues. PA1-761 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~23 kDa protein representing phospho AA-NAT in rat pineal gland whole protein extract. PA1-761 immunizing phosphopeptide (Cat. # PEP-064) corresponds to amino-terminal amino acid residues 24-33 of mouse AA-NAT. Total anti-amino-terminal AA-NAT-specific IgG (Cat. # PA1-760) was affinity purified on resin-coupled non-phosphopeptide (Cat. # PEP-063). Phospho-specific amino-terminal IgG (Cat. # PA1-761) was subsequently isolated on resin-coupled phosphopeptide (Cat. # PEP-064). This sequence is completely conserved between mouse, rat, and human AA-NAT protein.

Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatonin synthesis. Hormonal melatonin has been implicated in sleep and circadian clock function. Large changes in the abundance of AANAT are believed to be responsible for the large day/night rhythms in melatonin synthesis in the pineal gland and retina. Most research shows that AANAT activity is regulated by controlling the steady-state levels of the protein. Recent research, however, suggests that another mechanism may also play a role in controlling melatonin production without altering AANAT protein and activity. This mechanism may explain the sudden physiological increase in circulating melatonin observed at the onset of the dark period in humans.

Specifications

• Antigen: Phospho-AANAT (Thr29)
• Applications: Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA and 0.05% sodium azide
• Gene: Aanat
• Gene Accession No.: Q64666
• Gene Alias: Aanat; AA-NAT; Aralkylamine N-acetyltransferase; arylakylamine N-acetyltransferase; Arylalkylamine N - acetyltransferase (Serotonin N - acetyltransferase); arylalkylamine N-acetyltransferase; DSPS; Nat-2; Nat4; Seretonin N-acetyltransferase; serotonin acetylase; Serotonin N-acetyltransferase; SNAT
• Gene Symbols: Aanat
• Host Species: Rabbit
• Immunogen: Synthetic Peptide: C(24) Q R R H T(p) L P A S(33).
• Purification Method: Antigen affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 25120
• Target Species: Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov