Invitrogen™ Phospho-AGO2 (Ser387) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143647	100 μL

Catalog No. PIPA5143647 Supplier Invitrogen™ Supplier No. PA5143647

Description

Rabbit Polyclonal Antibody

This antibody was cross-absorbed to unphospho-Ago2 (Ser-387), before affinity purification using phospho-Ago2 (Ser-387) peptide (without carrier). The antibody detects a 97 kDa* protein corresponding to the apparent molecular mass of phosphorylated Ago2 on SDS-PAGE immunoblots of human A431 cells treated with Calyculin A and mouse recombinant Ago2 full length protein. This reactivity is not observed after lambda phosphatase dephosphorylation.

Ago proteins are broadly expressed in somatic cells, associate with miRNAs and are key actors in different RNA silencing pathways. However, only the Ago2 protein displays endonucleolytic or Slicer activity and can therefore execute miRNA-directed cleavage of target mRNA, provided that the base-pairing between the Ago2-associated miRNA and the mRNA sequence is perfect. In case of partial complementarity, the Ago2 protein fails to cleave, but instead interferes with translation of the target mRNA via its translational repression activity. Ago2 has been shown to play a non-redundant role in small RNA guided gene silencing processes, including RNA interference, translation repression and heterochromatinization. As a core element of the RISC complex, AGO2 initiates the degradation of target mRNAs through its catalytic activity in gene silencing processes guided by siRNAs or miRNAs. In addition to mRNA degradation or gene silencing guided by miRNAs, Ago2 also acts as a RNA slicer in a Dicer-independent way, as well as a regulator of miRNA maturation. Over-expression of Ago2 has been correlated with several aspects of cancers, including tumor cell growth and the overall survival of cancer patients. Furthermore, gene disruption in the mouse demonstrated that the Ago2 protein is essential for embryonic development and a key regulator of B-lymphoid and erythroid development.

Specifications

• Antigen: Phospho-AGO2 (Ser387)
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: AGO2
• Gene Accession No.: Q8CJG0, Q9QZ81, Q9UKV8
• Gene Alias: 1110029L17Rik; 2310051F07Rik; AGO1; AGO2; AI225898; AL022874; argonaute 2; argonaute 2, RISC catalytic component; argonaute RISC catalytic component 2; argonaute RISC catalytic subunit 2; Argonaute1; argonaute2; argonaute2 {ECO:0000255; AW546247; CTA-204B4.6; eIF2C; eIF2C 1; eIF-2C 1; eIF2C 2; eIF-2C 2; eIF2C 2 {ECO:0000255; eIF-2C 2 {ECO:0000255; EIF2C1; Eif2c2; ENSMUSG00000072493; Eukaryotic translation initiation factor 2C 2; eukaryotic translation initiation factor 2C 2 {ECO:0000255; eukaryotic translation initiation factor 2C, 2; GERp95; Gm10365; Golgi ER protein 95 kDa; hAgo1; hAgo2; HAMAP-Rule:MF_03031}; Kiaa4215; mAgo2; mKIAA4215; PAZ Piwi domain protein; Piwi/Argonaute family protein meIF2C2; PPD; Protein argonaute-1; protein argonaute-2; Protein slicer; Q10
• Gene Symbols: AGO2
• Host Species: Rabbit
• Immunogen: Phospho-Ago2 (Ser-387) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 387 in human Ago2. This peptide sequence is highly conserved in mouse, rat, chick, and fish Ago2. The site is also conserved in Ago1 and Ago4, but the homology surrounding the site is low between Ago2 and these two family members.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 239528, 27161, 59117
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG