Phospho-AKT1 (Ser129) Rabbit anti-Human, Polyclonal, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA577854

Catalog No. PIPA577854

Description

Positive Control: 293T, JC, BCL-1, PC-12, *mouse melanoma Predicted Reactivity: Zebrafish (93%), Xenopus laevis (99%), Cat (98%), Pig (99%), Chicken (100%), Sheep (99%), Rhesus Monkey (100%), Bovine (99%)

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.

Specifications

• Antigen: Phospho-AKT1 (Ser129)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: AKT1
• Gene Symbols: AKT1
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human AKT1 (phospho Ser129). The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Concentration: 0.82 mg/ml
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% Proclin 300; pH 7
• Gene Accession No.: P31749
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 207
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid