Invitrogen™ Phospho-AMPK alpha-1,2 (Thr172, Thr183) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA585649

Catalog No. PIPA585649

Description

Keep as concentrated solution. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Bovine (100%). Positive Control: MCF-7(100uM CCCP treatment for 2 hr), HeLa(500 uM CoCl2 treatment for 22 hr and 10 uM MG132 treatment for 2 hr). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Specifications

• Antigen: Phospho-AMPK alpha-1,2 (Thr172, Thr183)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRKAA1
• Gene Alias: 2310008I11Rik; 5'-AMP-activated protein kinase alpha-1 catalytic subunit; 5'-AMP-activated protein kinase alpha-2 catalytic subunit; 5'-AMP-activated protein kinase catalytic alpha-2 subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; 63 kDa subunit; A830082D05; AAPK2; ACACA kinase; acetyl-CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha 2; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK 63 kDa subunit; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 chain; AMPK subunit alpha-1; AMPK subunit alpha-2; AMPK1; AMPK2; AMPKa1; AMPKa2; AMPK-activated protein kinase alpha-2 subunit; AMPKalpha1; AMPK-alpha-1 chain; AMPKalpha2; AMPK-alpha-2 chain; C130083N04Rik; HMGCR kinase; hydroxymethylglutaryl-CoA reductase kinase; pAMPK; PRKAA; Prkaa1; Prkaa2; protein kinase; protein kinase AMP-activated alpha 2 catalytic subunit; protein kinase AMP-activated catalytic subunit alpha 1; protein kinase AMP-activated catalytic subunit alpha 2; protein kinase, AMP-activated, alpha 1 catalytic subunit; protein kinase, AMP-activated, alpha 2 catalytic subunit; tau-protein kinase PRKAA1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 105787, 108079, 5562, 5563
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1.5 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P54646, Q13131, Q5EG47, Q8BRK8
• Gene Symbols: PRKAA1, PRKAA2
• Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Thr183 of human AMPK alpha 1. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov