Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5121274

Catalog No. PIPA5121274

Description

Positive test controls include: 293T, C6. Immunogen sequence: LRTSC

The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Specifications

• Antigen: Phospho-AMPK alpha-1,2 (Thr183, Thr172)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PRKAA1
• Gene Alias: 2310008I11Rik; 5'-AMP-activated protein kinase alpha-1 catalytic subunit; 5'-AMP-activated protein kinase alpha-2 catalytic subunit; 5'-AMP-activated protein kinase catalytic alpha-2 subunit; 5'-AMP-activated protein kinase catalytic subunit alpha-1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; 5'-AMP-activated protein kinase, catalytic alpha-1 chain; 5'-AMP-activated protein kinase, catalytic alpha-2 chain; 63 kDa subunit; A830082D05; AAPK2; ACACA kinase; acetyl-CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase; AMP-activated protein kinase alpha 2; AMP-activated protein kinase alpha-2 subunit variant 2; AMP-activated protein kinase alpha-2 subunit variant 3; AMP-activated protein kinase alpha-2 variant B; AMP-activated protein kinase, alpha 1 catalytic subunit; AMP-activated protein kinase, catalytic, alpha-1; AMPK; AMPK 63 kDa subunit; AMPK alpha 1; AMPK alpha 2; AMPK alpha-1 chain; AMPK alpha-2 cha
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 105787, 108079, 5562, 5563, 65248, 78975
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1.48 mg/mL
• Formulation: PBS with 50% glycerol and 0.01% thimerosal; pH 7.3
• Gene Accession No.: P54645, P54646, Q09137, Q13131, Q5EG47, Q8BRK8
• Gene Symbols: PRKAA1, PRKAA2
• Immunogen: A synthetic phosphorylated peptide around T183 of human PRKAA1 (NP_0062425)
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG