Invitrogen™ Phospho-Ask1 (Ser83) Recombinant Rabbit Monoclonal Antibody (Ask1S83-G4)

Rabbit Recombinant Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA528020	Invitrogen™ MA528020	200 μL
PIMA536973	Invitrogen™ MA536973	20 μL

Description

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

MAP3K5 (ASK1) is a serine/threonine kinase that is abundantly expressed in human heart and pancreas and are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activates two different subgroups of MAPKK, MKK4 and MKK6, which in turn activates c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death and a catalytically inactive form of ASK1 inhibited TNF-a-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.

Specifications

• Antigen: Phospho-Ask1 (Ser83)
• Concentration: 0.5 mg/mL
• Applications: Flow Cytometry, Western Blot
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 0.1% BSA, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: O35099, Q99683
• Gene ID (Entrez): 26408, 365057, 4217
• Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Ser83 of human phospho Ask1.
• Product Type: Antibody

• Clone: Ask1S83-G4
• Content And Storage: -20°C
• Classification: Recombinant Monoclonal
• Form: Liquid
• Isotype: IgG κ
• Target Species: Human, Mouse, Rat
• Gene: MAP3K5
• Gene Alias: 7420452D20Rik; apoptosis signal regulating kinase 1; apoptosis signal-regulating kinase 1; ASK; ASK 1; ASK1; ASK-1; EC 2.7.11.25; kinase ASK1; M3K5; MAP/ERK kinase kinase 5; Map3k5; MAPK/ERK kinase kinase 5; MAPKKK5; MEK kinase 5; MEKK 5; Mekk5; mitogen activated protein kinase kinase kinase 5; mitogen-activated protein kinase kinase kinase 5; RGD1306565; RP3-325F22.4
• Gene Symbols: MAP3K5
• Primary or Secondary: Primary
• Purification Method: Protein A/G