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Invitrogen™ Phospho-ATF2 (Thr71) Polyclonal Antibody
Rabbit Polyclonal Antibody
Supplier: Invitrogen™ 44295G

Description
This antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non phosphorylated ATF2. The final product is generated by affinity chromatography using an ATF2-derived peptide phosphorylated at threonine 71. The peptide sequence is conserved in mouse, rat, chicken and frog. Suggested Western blot positive controls: NIH3T3 + anisomycin, or Jurkat + 10 μg/mL anisomycin for 60 minutes.
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2.
Specifications
Phospho-ATF2 (Thr71) | |
Polyclonal | |
Dulbecco's PBS with 1mg/mL BSA and 0.05% sodium azide; pH 7.3 | |
P15336 | |
Atf2 | |
Chemically synthesized phosphopeptide derived from a region of human ATF2 that contains threonine 71. | |
100 μL | |
Primary | |
Human | |
Antibody | |
IgG |
Immunohistochemistry (Paraffin), Western Blot | |
Unconjugated | |
Atf2 | |
Activating 2; Activating transcription factor 2; activating transcription factor 2 splice variant ATF2-var2; ATF2; Atf-2; cAMP response element-binding protein CRE-BP1; cAMP responsive element binding protein 2, formerly; cAMP-dependent transcription factor ATF-2; CREB2; CREB-2; CRE-BP; CREBP1; CRE-BP1; Cyclic AMP-dependent transcription factor ATF-2; cyclic AMP-responsive element-binding protein 2; D130078H02Rik; D18875; HB16; Histone acetyltransferase ATF2; MGC111558; mXBP; MXBP protein; OTTHUMP00000205345; OTTHUMP00000205347; OTTHUMP00000205348; OTTHUMP00000205350; OTTHUMP00000205352; OTTHUMP00000205353; OTTHUMP00000205354; Tg(Gzma-Klra1)7Wum; TREB7 | |
Rabbit | |
Antigen affinity chromatography | |
RUO | |
1386 | |
-20°C | |
Liquid |
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