Phospho-ATM (Ser1981) Monoclonal Antibody (10H11.E12), Rockland™

Mouse Monoclonal Antibody

Supplier:  Rockland Immunochemicals 200301400S

Catalog No. 50-199-0530

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Anti-ATM phospho S1981 Monoclonal Antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000; whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human ATM. Cross reactivity with ATM from other mammalian sources has not been tested. The immunogen has 91% sequence homology with mouse ATM.

Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

Specifications

• Antigen: Phospho-ATM (Ser1981)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: Q13315, Q62388
• Gene Symbols: ATM
• Immunogen: Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
• Regulatory Status: RUO
• Gene ID (Entrez): 11920, 300711, 472
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Immunofluorescence, Western Blot
• Clone: 10H11.E12
• Conjugate: Unconjugated
• Gene: ATM
• Gene Alias: AI256621; AT mutated; A-T mutated; A-T mutated homolog; AT mutated protein; AT1; ATA; Ataxia t; ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated homolog; ATC; ATD; ATDC; ATE; ATM; ATM (phospho S1981); ATM (pS1981); ATM (pSer1981); ATM phospho S1981; ATM phospho Ser1981; ATM serine/threonine kinase; C030026E19Rik; DKFZp781A0353; EC 2.7.1.37; MGC74674; phospho ATM; phospho S1981 ATM; Serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1
• Host Species: Mouse
• Quantity: 25 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG1 κ