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MilliporeSigma™ phospho-ATM (Ser1981), Mouse, Alexa Fluor 488, Clone: 10H11.E12,

Mouse Monoclonal Antibody

Manufacturer:  MilliporeSigma™ 05740AF488

Catalog No. 05-740-AF488


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Description

Description

Serine-protein kinase ATM (EC 2.7.11.1; UniProt Q13315; also known as A-T mutated, Ataxia telangiectasia mutated) is encoded by the ATM (also known as AT) gene (Gene ID 472) in human. Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair. Mutation in the ATM gene results in the autosomal recessive disease ataxia telangiectasia (AT). Known ATM substrates include p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1. The S/TQ sequence constitutes the essential substrates phosphorylation site motif. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate phosphorylation, while positively charged residues surrounding the S/TQ are negative determinants. The complex phenotypes of cells derived from patients with AT suggests that ATM has additional cellular substrates. ATM is present as an inactive homodimer or multimer prior to activation. DNA double-stranded breaks induced by ionizing radiation (IR) cause rapid ATM autophosphorylation at Ser1981 in human or the Ser1987 equivalent in mouse.
Specifications

Specifications

phospho-ATM (Ser1981)
10H11.E12
Alexa Fluor 488
ATM, AT
Purified
KLH-conjugated, synthetic peptide corresponding to amino acids 1974-1988 (SLAFEEG[pS]QSTTISS) of human ATM. The immunizing sequence is 11/12 identical to the corresponding mouse and rat sequence.
Protein G Purified
RUO
Stable for 1 year at 2°-8°C from date of receipt.
Monoclonal
Reacts with ATM phosphorylated at serine 1981. Clone 10H11.E12 is covered by US patent No. 6,916,627 and 7,108,992.
Immunocytochemistry
Please refer to lot specific datasheet.
Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % Sodium Azide.
Q13315
Mouse
IgG1κ
100μL
Epigenetics & Nuclear Function
Primary
NP_000042
Human, Mouse
Documents
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