Invitrogen™ Phospho-beta Arrestin 1 (Ser412) Monoclonal Antibody (H.992.9)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA515217

Catalog No. PIMA515217

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with beta-arrestin 2.

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold molecule, beta Arrestin 1 is essential for mitogenic signaling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosphorylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1 and 2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1 and 2 signaling pathway.

Specifications

• Antigen: Phospho-beta Arrestin 1 (Ser412)
• Classification: Monoclonal
• Conjugate: Unconjugated
• Gene: ARRB1
• Gene Alias: 1200006I17Rik; ARB1; ARR1; ARRB1; ARRB1 protein; arrdc1; arrestin; arrestin 2; arrestin beta 1; arrestin beta-1; arrestin, beta 1; arrestin-2; AW208571; BARRES; beta-arrestin; beta-arrestin1; beta-arrestin-1; G430100A01Rik; Non-visual arrestin-2
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 109689, 25387, 408
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot
• Clone: H.992.9
• Formulation: 0.01M HEPES with 100 μg/mL BSA, 50% glycerol, 0.15M NaCl and <0.02% sodium azide; pH 7.5
• Gene Accession No.: P29066, P49407, Q8BWG8
• Gene Symbols: ARRB1
• Immunogen: Synthetic peptide corresponding to residues surrounding Ser412 of human β-arrestin 1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat, Monkey
• Product Type: Antibody
• Isotype: IgG1