Phospho-beta Arrestin 1 (Ser412) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA538259

Catalog No. PIPA538259

Description

Beta Arrestin 1 is a member of a family of proteins widely expressed but especially abundant in the central nervous system. Serving as an adaptor or scaffold maolecule, beta Arrestin 1 is essential for mitogenic signalling and mediates agonist-dependent desensitization and internalization of Gprotein-coupled receptors (GPCRs, e.g., beta 2-adrenergic receptor). After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs are phosporylated by G-protein receptor kinases (GRKs) on serine residues. Beta Arrestin 1 in the cytosol is phosphorylated by ERK1&2 on serine412 in a negative feedback mechanism and binds to the phosphorylated receptors at the plasma membrane. Serine 412 is then dephosphorylated and the GPCRs are internalized, leading to activation of the Ras, Raf, ERK1&2 signaling pathway.

Specifications

• Antigen: Phospho-beta Arrestin 1 (Ser412)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ARRB1
• Gene Alias: ARRB1, ARR1, Beta-arrestin-1, Arrestin beta-1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 408
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: Dulbecco′s PBS with 150mM NaCl, 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P49407
• Gene Symbols: ARRB1
• Immunogen: A synthetic phosphopeptide derived from human Arrestin-1 around the phosphorylation site of Ser412 (T-G-SP-P-Q)
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Non-human Primate
• Product Type: Antibody
• Isotype: IgG