Invitrogen™ Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133) Polyclonal Antibody

Catalog No.	Quantity
PIPA5143664	100 μL

Catalog No. PIPA5143664 Supplier Invitrogen™ Supplier No. PA5143664

Description

Rabbit Polyclonal Antibody

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose before affinity purification using phospho-beta-Catenin (Tyr-142) peptide (without carrier). The antibody detects a 92kDa* protein corresponding to the molecular mass of beta-Catenin on SDS-PAGE immunoblots of Hct116 src-transformed cells treated with pervanadate, but not in control cells. Similar results were observed in pervanadate-treated A431 and human endothelial cells.

beta-Catenin is a 92 kDa protein that binds to the cytoplasmic tail of E-Cadherin. The cadherins, transmembrane adhesion molecules, are found with catenins at adherens junctions. Deletions in the cytoplasmic domain of E-Cadherin eliminate catenin binding and result in a loss of cell adhesion. Tyrosine phosphorylation of beta-Catenin can regulate its interaction with critical components of adherens junctions. Both Fer and Fyn kinases phosphorylate tyrosine 142 in vitro. Overexpression of these kinases in epithelial cells disrupts interactions between alpha- and beta-Catenins. The phosphorylation of tyrosine 142 may act as a switch from the transcriptional to the adhesive role of beta-Catenin. Src family kinases can also phosphorylate tyrosine 86 and 654 in beta-Catenin. The Tyr-654 phosphorylation regulates beta-Catenin binding to E-cadherin. Thus, site-specific tyrosine phosphorylation of beta-Catenin may regulate protein-protein interactions leading to changes in cell adhesion.

Specifications

• Antigen: Phospho-beta Catenin/gamma Catenin (Tyr142, Tyr133)
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.125 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: CTNNB1
• Gene Accession No.: P35222, Q02248, Q9WU82
• Gene Alias: Catenin beta1; CTNNB1
• Gene Symbols: CTNNB1
• Host Species: Rabbit
• Immunogen: Phospho-beta-Catenin (Tyr-142) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 142 of human beta-Catenin. This peptide sequence has one amino acid difference from a sequence around tyrosine 133 of human gamma-Catenin. These human sequences are highly conserved in rat and mouse beta- and gamma-Catenins.
• Purification Method: Antigen Affinity Chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12387, 1499, 84353
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG