Invitrogen™ Phospho-BLNK (Tyr84) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA126639

Catalog No. PA126639

Description

Recommended positive controls: EBI cells +/- H2O2 treatment. PA1-26639 is expected to cross react with chicken (83% conserved), mouse (100% conserved) due to sequence homology. The PA1-26639 immunogen is a synthetic phosphopeptide corresponding to a portion of human BLNK containing tyrosine 84. The sequence is conserved in mouse. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

B cell linker protein (Blnk) is a protein encoded by the Blnk gene located on chromosome 10 in humans. It is also known as SLP 65 (Src homology 2 domain-containing leukocyte protein of 65 kDa). It derives its name from being an adaptor protein in the B cell receptor signaling cascade with several supporting studies showing improper development of B cells in the absence of Blnk. Blnk undergoes tyrosine phosphorylation by Syk, a B cell receptor activated kinase, which provides a platform for interaction of signaling intermediaries such as the Vav GEF and Grb2.

Specifications

• Antigen: Phospho-BLNK (Tyr84)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: BLNK
• Gene Alias: AGM4; B cell adaptor containing SH2 domain; B cell linker; B cell linker protein; BASH; bca; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker; B-cell linker protein; Blnk; BLNK-S; Cytoplasmic adapter protein; LY57; Ly-57; lymphocyte antigen 57; Lyw-57; MGC111051; SLP65; Slp-65; Src homology [SH2] domain-containing leukocyte protein of 65 kD; Src homology 2 domain-containing leukocyte protein of 65 kDa
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17060, 29760
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: Q8WV28, Q9QUN3
• Gene Symbols: BLNK
• Immunogen: Synthetic phosphopeptide corresponding to a portion of human BLNK containing tyrosine 84. The sequence is conserved in mouse.
• Quantity: 50 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG