Phospho-BTK/ITK (Tyr551, Tyr511) Monoclonal Antibody (M4G3LN), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-9065	Invitrogen™ 17901541	25 Tests
50-112-3203	Invitrogen™ 17901542	100 Tests

Description

Description: This M4G3LN monoclonal antibody recognizes human and mouse Bruton's tyrosine kinase (also known as BTK, agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase)/interleukin-2-inducible tyrosine kinase (also known as ITK, T-cell-specific kinase, Tyrosine-protein kinase Lyk) when phosphorylated on Y551 and Y511, respectively. In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency. Specificity of this M4G3LN clone was determined by ELISA, flow cytometry, and western blotting. Applications Reported: This M4G3LN antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

In addition to Src family and SYK family kinases, B cells and T cells express a third family of receptor-proximal kinases called the Tec kinase family. The B cell-specific Tec family kinase is BTK; T cells express ITK as well as Tec and Rlk. Tec family kinases play an indispensable role in activation of phospholipase C and activation of calcium-dependent signaling cascades following antigen receptor triggering. BTK has been implicated in propagating signals downstream of LPS-TLR4 in B cells via signaling through the TLR4 co-receptor RP105/CD180. Mice congenitally lacking BTK or ITK show profound defects in B cell or T cell development and signaling, respectively. People with mutations or deletions in BTK suffer from X-linked agammaglobulinemia and X-linked immunodeficiency.

Specifications

• Antigen: Phospho-BTK/ITK (Tyr551, Tyr511)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: agammaglobulinaemia tyrosine kinase; agammaglobulinemia tyrosine kinase; AGMX1; AI528679; AT; ATK; B-cell progenitor kinase; BPK; Bruton agammaglobulinemia tyrosine kinase; bruton tyrosine kinase; Bruton's tyrosine kinase; BTK; dominant-negative kinase-deficient Brutons tyrosine kinase; Emt; homolog of mouse T-cell itk/tsk; IL2 inducible T cell kinase; IL2 inducible T-cell kinase; IL-2-inducible T cell kinase; IL-2-inducible T-cell kinase; IL2-inducible T-cell kinase; IMD1; interleukin-2-inducible T cell kinase; interleukin-2-inducible T-cell kinase; ITK; Kinase EMB; kinase EMT; Kinase TLK; LPFS1; LYK; PSCTK1; PSCTK2; T-cell-specific kinase; Tcsk; Tlk; truncated Bruton agammaglobulinemia tyrosine kinase; Tsk; Tyrosine-protein kinase BTK; Tyrosine-protein kinase BTK (Brutons tyrosine kinase) (Agammaglobulinaemia tyrosine kinase) (ATK) (B cell progenitor kinase) (BPK) (Kinase EMB); tyrosine-protein kinase BTK isoform (lacking exon 13 to 17); tyrosine-protein kinase BTK isoform (lacking exon 14); tyrosine-protein kinase ITK/TSK; Tyrosine-protein kinase Lyk; xid; XLA; X-linked immune deficiency
• Gene Symbols: BTK, ITK
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: ITK

• Clone: M4G3LN
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Mouse
• Target Species: Human, Mouse
• Gene Accession No.: P35991, Q03526, Q06187, Q08881
• Gene ID (Entrez): 12229, 16428, 3702, 695
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.