Phospho-C-Myb (Thr486) Polyclonal Antibody, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600401C47

Catalog No. 50-199-2013

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue. Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein.This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.

Transcriptional activator; DNA-binding protein that specifically recognize the sequence 5'-YAAC[GT]G-3'. Plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

Specifications

• Antigen: Phospho-C-Myb (Thr486)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: MYB
• Gene Alias: AI550390; Cmyb; c-myb; cmyb protein; c-myb_CDS; c-myb10A_CDS; c-myb13A_CDS; c-myb14A_CDS; c-myb8B_CDS; efg; gag-myb protein; M16449; Myb; myb proto-oncogene protein; MYB proto-oncogene, transcription factor; myeloblastosis oncogene; myeloblastosis proto-oncogene product; oncogene AMV; Proto-oncogene c-Myb; RP1-32B1.1; transcription factor cmyb; Transcriptional activator Myb; tumor-specific myb protein; v-myb avian myeloblastosis viral oncogene homolog
• Host Species: Rabbit
• Regulatory Status: RUO
• Gene ID (Entrez): 17863
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 0.91 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene Accession No.: P06876
• Gene Symbols: MYB
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody