Invitrogen™ Phospho-CD104 Integrin beta 4 (Tyr1494) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5143711

Catalog No. PIPA5143711

Description

This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-Integrin beta4 (Tyr-1494) peptide (without carrier). The antibody detects a 200kDa* protein corresponding to the molecular mass of Integrin beta4 on SDS-PAGE immunoblots of A431 cells treated with pervanadate, but not in control cells. Similar results were also observed in src-transformed Hct116 cells treated with pervanadate.

Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Mutations in this gene are associated with epidermolysis bullosa with pyloric atresia. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Specifications

• Antigen: Phospho-CD104 Integrin beta 4 (Tyr1494)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ITGB4
• Gene Alias: AA407042; C230078O20; CD antigen CD104; CD104; CD104 antigen; GP150; integrin beta 4; integrin beta-4; integrin subunit beta 4; integrin, beta 4; Itgb4
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 192897, 25724, 3691
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: ~0.25 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: A2A863, P16144, Q64632
• Gene Symbols: ITGB4
• Immunogen: Phospho-Integrin beta4 (Tyr-1494) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 1494 of human Integrin beta4. This peptide sequence is found in all three Integrin beta4 isoforms and is highly conserved in rat and mouse Integrin beta4.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG