Invitrogen™ Phospho-Cdc25A (Ser76) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536711

Catalog No. PIPA536711

Description

This antibody detects endogenous protein at a molecular weight of 59 and 70 kDa. Purity is >95% by SDS-PAGE.

CDC25a is a member of the CDC25 family of phosphatases. Three CDC25 genes are present in human cells: CDC25A, CDC25B, and CDC25C. These three genes function at different phases of the cell cycle. CDC25a is required for progression through G1 and S phases of the cell cycle. CDC25a activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated.

Specifications

• Antigen: Phospho-Cdc25A (Ser76)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: CDC25A
• Gene Alias: CDC25 isoform A1-CAG; CDC25A; CDC25A2; CDC25A2-CAG isoform; Cdc25m3; cell division cycle 25A; D9Ertd393e; dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1; MPIP1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 12530, 171102, 993
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: P30304, P48964, P48965
• Gene Symbols: CDC25A
• Immunogen: Synthetic phosphopeptide derived from human Cdc25A around the phosphorylation site of Serine 76.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG