Phospho-CDK4 (Thr172) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA5114560

Catalog No. PIPA5114560

Description

Antibody detects endogenous levels of CDK4 only when phosphorylated at Thr172.

CDK4/cyclin D3 serine/threonine kinase is dimeric protein complex. CDK4 is a member of the cyclin-dependent protein kinase family and is involved in the control of cell proliferation during the G1 phase of cell cycle. CDK4 forms a complex with the D-type cyclins and is inhibited by p16 (cyclin-dependent kinase inhibitor-2). CDK4 can mediate phosphorylation of the C-terminal region of Rb protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivity in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.

Specifications

• Antigen: Phospho-CDK4 (Thr172)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Cdk4
• Gene Alias: Cdk4; Cell division protein kinase 4; CMM3; Crk3; cyclin dependent kinase 4; cyclin-dependent kinase 4; MGC14458; p34<PSK-J3>/cdk4; PSK-J3; serine/threonine kinase; Unknown (protein for MGC:133903)
• Host Species: Rabbit
• Purification Method: sequential chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1019, 12567, 94201
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide
• Gene Accession No.: P11802, P30285, P35426
• Gene Symbols: Cdk4
• Immunogen: A synthesized peptide derived from human CDK4(Accession P11802), corresponding to amino acid residues around phosphorylated Thr172.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov