Phospho-CDK9 (Thr29)Rabbit anti-Human, Mouse, Rat, Polyclonal, Rockland™

Catalog No.	Quantity
50-194-3865	100 μg

Catalog No. 50-194-3865 Supplier Rockland Immunochemicals Supplier No. 600401996

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody is specific for human CDK9 protein phosphorylated at T29. A BLAST analysis was used to suggest cross-reactivity with CDK9 from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with CDK9 from other sources has not been determined.

CDK9 (cyclin-dependent kinase 9) is a member of the cyclin-dependent protein kinase family. It is involved in the regulation of transcription. CDK9 is a member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex. This complex facilitates the transition from abortive to productive elongation by phosphorylating the CTD of the large subunit of RNA polymerase II, POLR2A, SUPT5H, and RDBP. The complex is inactive when in the 7SK snRNP complex form. CDK9 phosphorylates EP300, MYOD1, RPB1/POLR2A, AR, DSIF, and NELF. It regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors. CDK9 promotes RNA synthesis in genetic programs for cell growth, differentiation, and viral pathogenesis. The CDK9/cyclin-K complex also has kinase activity towards CTD of RNAPII and can substitute for CDK9/cyclin-T P-TEFb in vitro. Chronic activation of CDK9 causes cardiac myocyte enlargement leading to cardiac hypertrophy and confers a predisposition to heart failure.

Specifications

• Antigen: Phospho-CDK9 (Thr29)
• Applications: ELISA, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1.3 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: CDK9
• Gene Accession No.: P50750, Q641Z4, Q99J95
• Gene Alias: BOS_12097; C-2K; CDC2L4; CDC2-related kinase; CDK9; Cell division cycle 2-like protein kinase 4; Cell division protein kinase 9; CTK1; cyclin dependent kinase 9; Cyclin-dependent kinase 9; cyclin-dependent kinase 9 (CDC2-related kinase); PITALRE; RP11-228B15.5; serine/threonine protein kinase PITALRE; Serine/threonine-protein kinase PITALRE; TAK; Tat-associated kinase complex catalytic subunit
• Gene Symbols: CDK9
• Host Species: Rabbit
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1025, 107951, 362110
• Target Species: Human, Mouse, Rat
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid