Invitrogen™ Phospho-CHK1 (Ser345) Recombinant Rabbit Monoclonal Antibody (PS01-17)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ MA545974

Catalog No. PIMA545974

Description

Predicted band size: 54 kDa Positive Control: HeLa treated with Etoposide cell lysate, HeLa treated with Hydroxyurea cell lysate, HeLa treated with 4mM Hydroxyurea for 20 hours. Subcellular Location: Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Sequence Similarities: 100% Mouse/Rat. Tissue Specificity: Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.

CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.

Specifications

• Antigen: Phospho-CHK1 (Ser345)
• Classification: Recombinant Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.1% BSA, 40% glycerol and 0.05% sodium azide
• Gene Accession No.: O14757
• Gene Symbols: Chek1
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding Ser345 of human Chk1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot, Immunocytochemistry
• Clone: PS01-17
• Conjugate: Unconjugated
• Gene: Chek1
• Gene Alias: C85740; Cell cycle checkpoint kinase; checkpoint kinase 1; checkpoint kinase 1 homolog; checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHEK1; CHK1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27; rad27 homolog; serine/threonine protein kinase CHK1; serine/threonine-protein kinase Chk1; Synoviolin; YBR1742; YBR274W
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 1111
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov