Invitrogen™ Phospho-CPI-17 (Thr38) Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA536842

Catalog No. PIPA536842

Description

This antibody detects endogenous protein at a molecular weight of 22 kDa. Purity is >95% by SDS-PAGE.

CPI-17 is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. CPI-17 was originally identified as a PKC-potentiated inhibitory protein of protein phosphatase-1, which is dominantly expressed in smooth muscle. Phosphorylation at Threonine 38, in vitro, by PKC or Rho-kinase enhances the inhibitory potency toward myosin phosphatase. CPI-17 is also phosphorylated at Threonine 38 by protein kinase N and might be involved in the calcium sensitization of smooth muscle contraction as a downstream effector of Rho and/or arachidonic acid. CPI-17 is dually phosphorylated at Serine 12 and Threonine 38 by a MYPT-associated kinase, M110 kinase.

Specifications

• Antigen: Phospho-CPI-17 (Thr38)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: PPP1R14A
• Gene Alias: 1110001M11Rik; 17 kDa PKC-potentiated inhibitory protein of PP1; 17-kDa PKC-potentiated inhibitory protein of PP1; 17-KDa protein; Cpi; CPI17; CPI-17; PKC-potentiated inhibitory protein of PP1; PPP1INL; PPP1R14A; Protein kinase C-potentiated inhibitor protein of 17 kDa; protein phosphatase 1 regulatory inhibitor subunit 14A; protein phosphatase 1 regulatory subunit 14A; protein phosphatase 1, regulatory (inhibitor) subunit 14A
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 114004, 68458, 94274
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: Q91VC7, Q96A00, Q99MC0
• Gene Symbols: PPP1R14A
• Immunogen: Synthetic phosphopeptide derived from human CPI-17 around the phosphorylation site of Threonine 38.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG