Invitrogen™ Phospho-CREB (Ser133) Recombinant Rabbit Monoclonal Antibody (B16H16L26)

Rabbit Recombinant Monoclonal Antibody

Supplier:  Invitrogen™ 700129

Catalog No. PI700129

Description

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

CREB (Cyclic AMP response element binding protein) is a 43 kDa basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation of CREB occurs via p44/42 MAP kinase and p90RSK, and via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB is involved in different cellular processes including the synchronization of circadian rhythmicity, differentiation of adipose cells, and learning and memory. In humans, the gene is located on the q arm of chromosome 2. Diseases associated with CREB dysfunction include Alzheimer's Disease, histiocytoma and soft tissue melanoma.

Specifications

• Antigen: Phospho-CREB (Ser133)
• Classification: Recombinant Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P16220, Q01147
• Gene Symbols: CREB1
• Immunogen: proprietary.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG

• Applications: Western Blot
• Clone: B16H16L26
• Conjugate: Unconjugated
• Gene: CREB1
• Gene Alias: 2310001E10Rik; 3526402H21Rik; active transcription factor CREB; AV083133; cAMP response element binding protein 1; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB; CREB1; Creb-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; cyclic AMP-responsive element-binding protein 1; htCREB; MGC9284; OTTHUMP00000206661; OTTHUMP00000206662; OTTHUMP00000206667; transactivator protein; Y protein
• Host Species: Rabbit
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 12912, 1385
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov